CM plus YE Agar, Modified

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CM plus YE Agar, Modified

培养基配方
Composition per 1001.0mL:
NaCl........................................................................................150.0g
MgSO4 ·7H2O.........................................................................20.0g
Agar.........................................................................................15.0g
Yeast extract............................................................................10.0g
Vitamin assay casamino acids ......................................................7.5g
Trisodium citrate·2H2O.............................................................3.0g
KCl...........................................................................................2.0g
Fe 2+ solution..........................................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Fe 2+ Solution:
Composition per 100.0mL:
FeSO4 ·7H2O...........................................................................4.98g
Preparation of Fe 2 + Solution:  Add FeSO4 ·7H2O to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except Fe 2+ solution, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 1.0mL
of sterile Fe 2+ solution. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use: For the cultivation of Actinopolyspora mortivallis.