CN Screen Medium (Cryptococcus neoformans Screen Medium)

[所属分类:培养基配方] [发布时间:2020-5-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CN Screen Medium
(Cryptococcus neoformans
Screen Medium)

培养基配方
Composition per liter:
Agar........................................................................15.0g
K2HPO4 ......................................................................4.0g
MgSO4 ·7H2O.................................................................2.5g
Glucose.....................................................................1.25g
Asparagine...................................................................1.0g
Glutamine ...................................................................1.0g
Glycine......................................................................1.0g
Thiamine·HCl ...............................................................1.0g
Tryptophan...................................................................1.0g
EDTA.........................................................................0.6g
Biotin......................................................................0.51g
Dihydroxyphenylalanine (Dopa)................................................0.2g
Phenol Red...................................................................0.2g
pH 5.5–5.6 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C.
Use: For the screening of yeast isolates for the presumptive identifica-
tion of Cryptococcus neoformans. Cryptococcus neoformans forms
black colonies.