CNS Agar

[所属分类:培养基配方] [发布时间:2020-5-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CNS Agar

培养基配方
Composition per liter:
Agar........................................................................15.0g
LiCl........................................................................10.0g
Pancreatic digest of gelatin.................................................5.0g
Beef extract.................................................................3.0g
K2HPO4 ......................................................................2.0g
Yeast extract................................................................2.0g
KH2PO4 ......................................................................0.5g
Glucose solution ..........................................................50.0mL
MgSO4 ·7H2O solution......................................................10.0mL
Antibiotic solution........................................................10.0mL
Bravo 500.................................................................0.082mL
pH 6.9 ± 0.2 at 25°C
Glucose Solution:
Composition per 50.0mL:
Glucose......................................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril-
ize.
MgSO4 ·7H2O Solution:
Composition per 10.0mL:
MgSO4 ·7H2O.................................................................0.25g
Preparation of MgSO4 ·7H2O Solution: Add MgSO4 ·7H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide.................................................................0.04g
Polymyxin B sulfate..........................................................0.032g
Nalidixic acid...............................................................0.025g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components—except glucose solu-
tion, MgSO4 ·7H2O solution, and antibiotic solution—to distilled/de-
ionized water and bring volume to 930.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile glucose
solution, 10.0mL of sterile MgSO4 ·7H2O solution, and 10.0mL of ster-
ile antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the isolation and cultivation of Corynbacterium nebraskense.