Coagulase Agar Base

[所属分类:培养基配方] [发布时间:2020-5-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Coagulase Agar Base

培养基配方
Composition per liter:
Agar .....................................................................25.0g
Brain heart infusion .....................................................10.5g
Pancreatic digest of casein...............................................10.5g
D- Mannitol...............................................................10.0g
Brain heart infusion ......................................................5.0g
NaCl.......................................................................3.5g
Papaic digest of soybean meal..............................................3.5g
Bromcresol Purple ........................................................0.02g
Rabbit plasma ..........................................................100.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium:  Add components, except rabbit plasma,
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat, while stirring, until boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Add rabbit plasma to a final concentration of 7–15%. Mix thoroughly.
Pour into sterile Petri dishes in 18.0mL volume per plate.
Use: For the cultivation and differentiation of Staphylococcus aureus
from other Staphylococcus species based on coagulase production.