Coagulase Mannitol Agar

[所属分类:培养基配方] [发布时间:2020-5-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Coagulase Mannitol Agar

培养基配方
Composition per liter:
Agar ....................................................................14.5g
Pancreatic digest of casein..............................................10.5g
D- Mannitol..............................................................10.0g
Brain heart infusion .....................................................5.0g
NaCl......................................................................3.5g
Papaic digest of soybean meal.............................................3.5g
Bromcresol Purple........................................................0.02g
Rabbit plasma with 0.15% EDTA..........................................100.0mL
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components, except rabbit plasma,
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat while stirring until boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. For
detection of coagulase activity add rabbit plasma with 0.15% EDTA to
a final concentration of 7–15%. Mix thoroughly. Pour into sterile Petri
dishes.
Use: For the cultivation and differentiation of Staphylococcus aureus
from other Staphylococcus species based on coagulase production and
mannitol fermentation.