Coagulase Mannitol HiVeg Agar Base with Plasma

[所属分类:培养基配方] [发布时间:2020-5-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Coagulase Mannitol HiVeg Agar Base with Plasma

培养基配方
Composition per liter:
Agar.......................................................................14.5g
Plant hydrolysate .........................................................10.5g
Mannitol...................................................................10.0g
Plant special infusion......................................................5.0g
Papaic digest of soybean meal...............................................3.5g
NaCl........................................................................3.5g
Bromcresol Purple...........................................................0.02
Rabbit plasma.....................................................100.0–150.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components, except rabbit plasma,
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat while stirring until boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. For
detection of coagulase activity add rabbit plasma with 0.15% EDTA to
a final concentration of 7–15%. Mix thoroughly. Pour into sterile Petri
dishes.
Use: For the cultivation and differentiation of Staphylococcus aureus
from other Staphylococcus species based on coagulase production and
mannitol fermentation. For the primary isolation and identification of
pathogenic Staphylococci from clinical specimens or for classifying
pure cultures.