COBA (Colistin Oxolinic Acid Blood Agar)

[所属分类:培养基配方] [发布时间:2020-5-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

COBA
(Colistin Oxolinic Acid Blood Agar)

培养基配方
Composition per liter:
Columbia agar base.......................................................930.0mL
Horse blood, defibrinated, sterile........................................50.0mL
Colistin sulfate solution.................................................10.0mL
Oxolinic acid solution....................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Columbia Agar Base:
Composition per 930.0mL:
Agar........................................................................13.5g
Pancreatic digest of casein.................................................10.0g
Peptic digest of animal tissue .............................................10.0g
NaCl.........................................................................5.0g
Beef extract.................................................................3.0g
Yeast extract................................................................3.0g
Cornstarch...................................................................1.0g
Preparation of Columbia Agar Base:  Add components to dis-
tilled/deionized water and bring volume to 930.0mL. Mix thoroughly.
Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Colistin Sulfate Solution:
Composition per 10.0mL:
Colistin sulfate...........................................................10.0mg
Preparation of Colistin Sulfate Solution: Add colistin sulfate to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Oxolinic Acid Solution:
Composition per 10.0mL:
Oxolinic acid..........................................................5.0–10.0mg
Preparation of Oxolinic Acid Solution: Add oxolinic acid to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: To 930.0mL of sterile, cooled Columbia
agar base, add sterile colistin sulfate, sterile oxolinic acid, and sterile, de-
fibrinated horse blood. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation and cultivation of streptococci in pure culture
from mixed flora in clinical specimens.