​Colby and Zatman Agar

[所属分类:培养基配方] [发布时间:2020-5-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Colby and Zatman Agar

培养基配方
Composition per liter:
Agar, noble...............................................................20.0g
K2HPO4 ....................................................................1.2g
KH2PO4 ...................................................................0.62g
(NH4)2SO4 .................................................................0.5g
MgSO4 ·7H2O...............................................................0.2g
NaCl.......................................................................0.1g
CaCl2 ·6H2O..............................................................0.05g
ZnSO4 ·7H2O............................................................70.0μg
H3BO3 ..................................................................10.0μg
MnSO4 ·5H2O............................................................10.0μg
Na2MoO4 ·2H2O .........................................................10.0μg
CoCl2 ·6H2O ............................................................5.0μg
CuSO4 ·5H2O.............................................................5.0μg
FeCl3 ·6H2O..............................................................1.0mg
Trimethylamine solution..................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Trimethylamine Solution
Composition per 10.0mL:
Trimethylamine............................................................1.0g
Preparation of Trimethylamine Solution : Add trimethylamine
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except trimethylamine
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 10.0mL of sterile trimethylamine solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of Aminobacter aminovorans, Bacillus spe-
cies, Hyphomicrobium aestuarii, Hyphomicrobium facilis, Hyphomi-
crobium species, Hyphomicrobium variabile, Hyphomicrobium zavar-
zinii, Methylobacterium extorquens, Methylobacterium species, and
Methylophilus methylotrophus.