​Colby and Zatman Medium

[所属分类:培养基配方] [发布时间:2020-5-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Colby and Zatman Medium

培养基配方
Composition per liter:
Agar .......................................................................15.0g
K2HPO4 ......................................................................1.2g
KH2PO4 .....................................................................0.62g
(NH4)2SO4 ...................................................................0.5g
MgSO4 ·7H2O.................................................................0.2g
NaCl.........................................................................0.1g
CaCl2 ·2H2O...............................................................34.0mg
FeCl3 ·H2O.................................................................1.0mg
Trace elements solution .....................................................1.0mL
Trimethylamine,
10% solution, filter sterilized ............................................10.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
ZnSO4 ·7H2O.................................................................70.0mg
H3BO3 .......................................................................10.0mg
Na2MoO4 ·2H2O...............................................................10.0mg
MnSO4 ·H2O...................................................................7.0mg
CoCl2 ·H2O...................................................................5.0mg
CuSO4 ·5H2O..................................................................5.0mg
Preparation of Medium: Add components, except trimethylamine
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL
of sterile trimethylamine solution. Mix thoroughly. Pour into sterile Pe-
tri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Aminomonas aminovorus.