Colby and Zatman Thiamine Medium

[所属分类:培养基配方] [发布时间:2020-5-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Colby and Zatman Thiamine Medium

培养基配方
Composition per liter:
Agar, noble..................................................................20.0g
K2HPO4 .......................................................................1.2g
KH2PO4 ......................................................................0.62g
(NH4)2 ·SO4 .................................................................0.5g
MgSO4 ·7H2O..................................................................0.2g
NaCl..........................................................................0.1g
CaCl2 ·6H2O ................................................................0.05g
FeCl3 ·6H2O.................................................................1.0mg
ZnSO4 ·7H2O...............................................................70.0μg
H3BO3 .....................................................................10.0μg
MnSO4 ·5H2O...............................................................10.0μg
Na2MoO4 ·2H2O.............................................................10.0μg
CoCl2 ·6H2O................................................................5.0μg
CuSO4 ·5H2O................................................................5.0μg
Thiamine.....................................................................0.5mg
Trimethylamine solution.....................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Trimethylamine Solution
Composition per 10.0mL:
Trimethylamine...............................................................1.0g
Preparation of Trimethylamine Solution : Add trimethylamine
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except trimethylamine
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 10.0mL of sterile trimethylamine solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of Aminobacter aminovorans, Bacillus spe-
cies, Hyphomicrobium aestuarii, Hyphomicrobium facilis, Hyphomi-
crobium species, Hyphomicrobium variabile, Hyphomicrobium zavar-
zinii, Methylobacterium extorquens, Methylobacterium species, and
Methylophilus methylotrophus.