Colonization Medium

[所属分类:培养基配方] [发布时间:2020-5-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Colonization Medium

培养基配方
Composition per 700.0mL:
Mannose.................................................................1.0g
Pancreatic digest of gelatin............................................0.2g
Brain heart, solids from infusion .....................................0.08g
Peptic digest of animal tissue.........................................0.08g
NaCl...................................................................0.07g
Glucose ...............................................................0.04g
Na2HPO4 ...............................................................0.03g
Bile salts No.3.........................................................0.1g
Dulbecco’s phosphate-buffered saline................................700.0mL
pH 7.4 ± 0.2 at 25°C
Dulbecco’s Phosphate-Buffered Saline:
Composition per liter:
NaCl...................................................................8.0g
Na2HPO4 ·7H2O........................................................2.16g
KCl....................................................................0.2g
KH2PO4 ................................................................0.2g
CaCl2 .................................................................0.1g
MnCl2 ·6H2O ..........................................................0.1g
Preparation of Dulbecco’s Phosphate-Buffered Saline: Add
components to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly.
Preparation of Medium:  Combine components. Mix thoroughly.
Filter sterilize. Aseptically distribute into sterile tubes or flasks.
Use: For the differentiation of enterotoxigenic Escherichia coli from
foods based on the HeLa cell test for colonization.
Colonization Medium with Dulbecco’s Phosphate Buffer
Composition per liter:
NaCl.....................................................................8.0g
Mannose..................................................................1.4g
K2HPO4 .................................................................1.15g
Brain heart infusion powder .............................................0.7g
KCl......................................................................0.2g
KH2PO4 ..................................................................0.2g
Bile salts mixture......................................................0.14g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Do
not autoclave or heat. Aseptically distribute into tubes.
Use: For preparation of the solution of enterotoxigenic Escherichia
coli used for colonization test in HeLa cell lines.