Columbia Agar

[所属分类:培养基配方] [发布时间:2020-5-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia Agar

培养基配方
Composition per liter:
Columbia agar base........................................................950.0mL
Sheep blood ..............................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Columbia Agar Base:
Composition per liter:
Agar.......................................................................13.5g
Pancreatic digest of casein................................................12.0g
NaCl........................................................................5.0g
Peptic digest of animal tissue .............................................5.0g
Beef extract................................................................3.0g
Yeast extract...............................................................3.0g
Cornstarch..................................................................1.0g
Preparation of Columbia Agar Base: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C.
Preparation of Medium: To 950.0mL of cooled, sterile Columbia
agar base, aseptically add 50.0mL of sterile, defibrinated sheep blood. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of nonfastidious and fastidious
microorganisms from a variety of clinical and nonclinical specimens.