Columbia Blood Agar

[所属分类:培养基配方] [发布时间:2020-5-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia Blood Agar

培养基配方
Composition per liter:
Columbia blood agar base ................................................950.0mL
Sheep blood ..............................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Columbia Blood Agar Base:
Composition per liter:
Agar .....................................................................15.0g
Pantone...................................................................10.0g
Bitone....................................................................10.0g
NaCl.......................................................................5.0g
Tryptic digest of beef heart...............................................3.0g
Cornstarch.................................................................1.0g
Source: Columbia blood agar base is available as a premixed powder
from BD Diagnostic Systems.
Preparation of Columbia Blood Agar Base: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Preparation of Medium: To 950.0mL of cooled, sterile Columbia
blood agar base, aseptically add 50.0mL of sterile, defibrinated sheep
blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: With the addition of blood or other enrichments, used for the iso-
lation and cultivation of fastidious microorganisms.