Columbia Blood Agar (DSMZ Medium 693)

[所属分类:培养基配方] [发布时间:2020-5-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia Blood Agar
(DSMZ Medium 693)

培养基配方
Composition per liter:
Columbia blood agar base..............................................950.0mL
Sheep blood............................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Columbia Blood Agar Base:
Composition per liter:
Special peptone........................................................23.0g
Agar ..................................................................10.0g
NaCl....................................................................5.0g
Starch .................................................................1.0g
Preparation of Columbia Blood Agar Base: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Preparation of Medium: To 950.0mL of cooled, sterile Columbia
blood agar base, aseptically add 50.0mL of sterile, defibrinated sheep
blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation of Corynebacterium spp., Actinomyces spp.,
Arcanobacterium spp., Streptococcus pneumoniae, Lactobacillus
iners, Isobaculum melis, Nocardia paucivorans, and a variety of fastid-
ious microorganisms.