Desulfovibrio MG-1 Medium (DSMZ Medium 615)

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio MG-1 Medium
(DSMZ Medium 615)

培养基配方
Composition per liter:
Na2SO4 .......................................................................4.5g
Glycerol .....................................................................2.0g
NH4Cl ........................................................................1.0g
Yeast extract.................................................................1.0g
Na3 -citrate·2H2O............................................................0.6g
KH2PO4 .......................................................................0.5g
Na-thioglycolate..............................................................0.1g
MgSO4 ·7H2O.................................................................0.06g
CaCl2 ·2H2O.................................................................0.04g
FeSO4 ·7H2O.................................................................4.0mg
Resazurin ...................................................................0.5mg
pH 6.9 ± 0.2 at 25°C
Preparation of Medium: Prepare and dispense medium under 100%
N2 gas atmosphere. Add components to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Desulfovibrio sp.