Desulfovibrio MG-1 Medium

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio MG-1 Medium

培养基配方
Composition per liter:
Na2SO4 .......................................................................4.5g
Glycerol .....................................................................2.0g
NH4Cl ........................................................................1.0g
Yeast extract.................................................................1.0g
Trisodium citrate·2H2O.......................................................0.6g
KH2PO4 .......................................................................0.5g
Sodium thioglycolate..........................................................0.1g
MgSO4 ·7H2O.................................................................0.06g
CaCl2 ·2H2O ................................................................0.04g
FeSO4 ·7H2O.................................................................4.0mg
Resazurin....................................................................0.5mg
pH 7.5 ± 0.2 at 25°C
Preparation of Medium:  Prepare and dispense medium under
100% N2 . Add components to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Contin-
ue boiling for 5 min. Cool to room temperature while sparging with
100% N2 . Anaerobically distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For the cultivation of Desulfovibrio species.