Desulfurococcus Medium

[所属分类:培养基配方] [发布时间:2020-6-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfurococcus Medium

培养基配方
Composition per 1300.0mL:
Solution C..............................................................500.0mL
Solution B..............................................................450.0mL
Solution A..............................................................300.0mL
Solution D...............................................................50.0mL
Solution A:
Composition per 300.0mL:
(NH4)2SO4 ...............................................................1.3g
KH2PO4 .................................................................0.28g
MgSO4 ·7H2O............................................................0.25g
CaCl2 ·2H2O ...........................................................0.07g
FeSO4 ·7H2O...........................................................0.028g
Na2B4O7 ·10H2O.........................................................4.5mg
MnCl2 ·4H2O ...........................................................1.8mg
ZnSO4 ·7H2O...........................................................0.22mg
CuCl2 ·2H2O...........................................................0.05mg
Na2MoO4 ·2H2O.........................................................0.03mg
VOSO4 ·2H2O...........................................................0.03mg
CoSO4 ·7H2O...........................................................0.01mg
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 300.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 25°C. Gas under 100% N2 for 20 min.
Solution B:
Composition per 450.0mL:
Sulfur...................................................................5.0g
Preparation of Solution B: Add sulfur to distilled/deionized water
and bring volume to 450.0mL. Autoclave for 30 min at 0 psi pressure–
100°C on 3 consecutive days. Gas under 100% N2 for 20 min.
Solution C:
Composition per 500.0mL:
Pancreatic digest of casein..............................................2.0g
Yeast extract............................................................2.0g
Resazurin...............................................................1.0mg
Preparation of Solution C: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 25°C. Gas under 100% N2 for 20 min.
Solution D:
Composition per 50.0mL:
Na2S·9H2O................................................................0.5g
Preparation of Solution D: Add Na2S·9H2O to distilled/deionized
water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 25°C. Gas under 100% N2 for 20 min.
Preparation of Medium: Aseptically combine solutions A–D un-
der nitrogen gas. Seal containers with butyl rubber stoppers.
Use:  For the cultivation and maintenance of Desulfurococcus mobilis
and Desulfurococcus mucosus.