Desulfurococcus Medium (DSMZ Medium 184)

[所属分类:培养基配方] [发布时间:2020-6-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfurococcus Medium
(DSMZ Medium 184)

培养基配方
Composition per liter:
Sulfur, powdered...............................................................5.0g
(NH4)2SO4 .....................................................................1.3g
Yeast extract..................................................................1.0g
KH2PO4 .......................................................................0.28g
MgSO4 ·7H2O..................................................................0.25g
CaCl2 ·2H2O..................................................................0.07g
FeCl3 ·6H2O..................................................................0.02g
Na2B4O7 ·10H2O...............................................................4.5mg
MnCl2 ·4H2O..................................................................1.8mg
Resazurin ....................................................................1.0mg
ZnSO4 ·7H2O.................................................................0.22mg
CuCl2 ·2H2O ................................................................0.05mg
Na2MoO4 ·4H2O ..............................................................0.03mg
VOSO4 ·2H2O.................................................................0.03mg
CoSO4 .......................................................................0.01mg
Na2S·9H2O solution...........................................................10.0mL
pH 5.8 ± 0.2 at 25°C
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O.....................................................................0.5g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Sulfur: Add 10.0g of powdered sulfur to a flask and
sterilize by steaming for 3 hr on 3 consecutive days.
Preparation of Medium: Prepare and dispense medium under
100% N2 . Add components, except sulfur, and Na2S·9H2O solution, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 5.0g of sterile sulfur and 10.0mL of sterile
Na2S·9H2O solution. Adjust pH to 5.5 with 10N sterile H2SO4 . Mix
thoroughly. Aseptically and anaerobically distribute into sterile tubes
or flasks.
Use: For the cultivation of Desulfurococcus mobilis and Desulfuro-
coccus mucosus.