Desulfurococcus Medium

[所属分类:培养基配方] [发布时间:2020-6-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfurococcus Medium

培养基配方
Composition per 1010.0mL:
Sulfur, powdered.............................................................5.0g
(NH4)2SO4 ...................................................................1.3g
Yeast extract................................................................1.0g
KH2PO4 .....................................................................0.28g
MgSO4 ·7H2O................................................................0.25g
CaCl2 ·2H2O ...............................................................0.07g
FeCl3 ·6H2O................................................................0.02g
Na2B4 ·10H2O...............................................................4.5mg
MnCl2 ·4H2O ...............................................................1.8mg
Resazurin ..................................................................1.0mg
ZnSO4 ·7H2O...............................................................0.22mg
CuCl2 ·2H2O...............................................................0.05mg
Na2MoO4 ·2H2O.............................................................0.03mg
VOSO4 ·2H2O...............................................................0.03mg
CoSO4 .....................................................................0.01mg
Na2S·9H2O solution........................................................10.0mL
pH 5.5 ± 0.2 at 25°C
Preparation of Sulfur: Sterilize 5.0g of powdered sulfur by steam-
ing for 3 hr on 3 consecutive days.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O.................................................................0.5g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except Na2S·9H2O so-
lution and sulfur, to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Sparge with 100% N2 . Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically and anaerobically add 10.0mL of sterile
Na2S·9H2O solution and 5.0g of sterile powdered sulfur. Mix thor-
oughly. Final pH of medium should be 5.5.
Use:  For the cultivation and maintenance of Desulfurococcus mobilis
and Desulfurococcus mucosus.