Desulfuromonas spp. Medium (DSMZ Medium 148)

[所属分类:培养基配方] [发布时间:2020-6-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfuromonas spp. Medium
(DSMZ Medium 148)

培养基配方
Composition per 1001.0mL:
Fumaric acid ................................................................1.5g
KH2PO4 ......................................................................1.0g
NH4Cl .......................................................................0.5g
Na-acetate...................................................................0.5g
MgSO4 ·7H2O.................................................................0.4g
CaCl2 ·2H2O ................................................................0.1g
Resazurin ..................................................................0.5mg
NaHCO3 solution.............................................................40.0mL
Trace elements solution SL-4................................................10.0mL
Na2S·9H2O solution..........................................................6.0mL
Vitamin solution.............................................................5.0mL
pH 7.5 ± 0.2 at 25°C
NaHCO3 Solution:
Composition per 100.0mL:
NaHCO3 .......................................................................5.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Must be
prepared freshly.
Trace Elements Solution SL-4:
Composition per liter:
EDTA...........................................................................0.5g
FeSO4 ·7H2O...................................................................0.2g
Trace elements solution SL-6................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H3BO3 ......................................................................0.3g
CoCl2 ·6H2O................................................................0.2g
ZnSO4 ·7H2O................................................................0.1g
MnCl2 ·4H2O ..............................................................0.03g
Na2MoO4 ·H2O..............................................................0.03g
NiCl2 ·6H2O...............................................................0.02g
CuCl2· ·2H2O.............................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl...........................................................62.5mg
Nicotinic acid...........................................................25.0mg
Thiamine-HCl·2H2O.......................................................12.5mg
p-Aminobenzoic acid......................................................12.5mg
Ca-pantothenate...........................................................6.5mg
Biotin ...................................................................2.5mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Na2S·9H2O Solution:
Composition per 100.0mL:
Na2S·9H2O................................................................5.0g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Before use, neutralize to pH 7.0 with sterile HCl.
Preparation of Medium: Add components, except vitamin solution,
NaHCO 3 solution, and Na2S·9H2O solution, to 950.0mL distilled/de-
ionized water. Adjust pH to 5.0. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically and anaerobically add 5.0mL vitamin solution,
40.0mL NaHCO 3 solution, and 6.0mL Na2S·9H2O solution. Mix thor-
oughly. Adjust pH to 7.5 with sterile 2MNa2CO3 and 2MH2SO4 , re-
spectively. Aseptically distribute into sterile screw-capped bottles or
tubes. Fill completely, leaving only a small gas bubble.
Use: For the cultivation of Desulfuromonas acetexigenes.