Desulfuromonas succinoxidans Medium

[所属分类:培养基配方] [发布时间:2020-6-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfuromonas succinoxidans Medium

培养基配方
Composition per liter:
NaCl.......................................................................20.0g
MgCl2 ·6H2O................................................................2.0g
KH2PO4 .....................................................................1.0g
MgSO4 ·7H2O................................................................1.0g
NH4Cl ......................................................................0.3g
CaCl2 ·2H2O................................................................0.1g
Resazurin .................................................................0.5mg
NaHCO3  solution .........................................................50.0mL
Disodium fumarate solution................................................10.0mL
Sodium acetate solution...................................................10.0mL
Na2S·9H2O solution.......................................................10.0mL
Trace elements solution SL-10 .............................................1.0mL
Seven vitamin solution.....................................................1.0mL
pH 7.2 ± 0.2 at 25°C
NaHCO3 Solution:
Composition per 50.0mL:
NaHCO3 .....................................................................2.5g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 50.0mL. Mix thoroughly. Sparge
with 80% N2 + 20% CO2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Disodium Fumarate Solution:
Composition per 10.0mL:
Disodium fumarate ..........................................................1.6g
Preparation of Disodium Fumarate Solution:  Add disodium
fumarate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Sparge with 100% N2 . Autoclave for 15 min at 15 psi pres-
sure–121°C.
Sodium Acetate Solution:
Composition per 10.0mL:
Sodium acetate...............................................................0.8g
Preparation of Sodium Acetate Solution: Add sodium acetate to
distilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O.....................................................................0.5g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O..................................................................1.5g
CoCl2 ·6H2O...............................................................190.0mg
MnCl2 ·4H2O ..............................................................100.0mg
ZnCl2 ......................................................................70.0mg
Na2MoO4 ·2H2O..............................................................36.0mg
NiCl2 ·6H2O................................................................24.0mg
H3BO3 .......................................................................6.0mg
CuCl2 ·2H2O.................................................................2.0mg
HCl (25% solution)..........................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add
FeCl2 ·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/
deionized water and bring volume to 1.0L. Add remaining compo-
nents. Mix thoroughly.
Seven Vitamin Solution:
Composition per liter:
Pyridoxine·HCl................................................................0.3g
Thiamine·HCl..................................................................0.2g
Nicotinic acid.................................................................0.2g
Calcium  DL -pantothenate......................................................0.1g
Vitamin B 12 ..................................................................0.1g
p-Aminobenzoic acid..........................................................80.0mg
Biotin.......................................................................20.0mg
Preparation of Seven Vitamin Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize. Sparge with 100% N2 .
Preparation of Medium: Prepare and dispense medium under 80%
N2 + 20% CO2 . Add components, except NaHCO3  solution, disodium
fumarate solution, sodium acetate solution, Na2S·9H2O solution, and
seven vitamin solution, to distilled/deionized water and bring volume
to 920.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2 . Auto-
clave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobi-
cally add 50.0mL of sterile NaHCO3  solution, 10.0mL of sterile
disodium fumarate solution, 10.0mL of sterile sodium acetate solution,
10.0mL of sterile Na2S·9H2O solution, and 1.0mL of sterile seven vi-
tamin solution. Mix thoroughly.
Use: For the cultivation of Desulfuromonas succinoxidans.