Desultobacterium Medium (DSMZ Medium 383)

[所属分类:培养基配方] [发布时间:2020-6-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desultobacterium Medium
(DSMZ Medium 383)

培养基配方
Composition per 1012.0mL:
Soultion A...............................................................930.0mL
Solution C................................................................50.0mL
Solution D................................................................10.0mL
Solution E ...............................................................10.0mL
Solution G................................................................10.0mL
Solution B.................................................................1.0mL
Solution F.................................................................1.0mL
pH 7.0 at 25°C
Solution A:
Composition per 930.0mL:
NaCl......................................................................21.0g
Na2SO4 ...................................................................3.0g
MgCl2 ·6H2O..............................................................3.0g
KCl.......................................................................0.5g
NH4Cl ....................................................................0.3g
KH2PO4 ...................................................................0.2g
CaCl2 ·2H2O.............................................................0.15g
Resazurin ...............................................................1.0mg
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 930.0mL. Mix thoroughly. Sparge with 80%
N2  + 20% CO2 gas until saturated. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C.
Solution B:
Composition per liter:
FeCl2 ·4H2O................................................................1.5g
CoCl2 ·6H2O ............................................................190.0mg
MnCl2 ·4H2O.............................................................100.0mg
ZnCl2 ...................................................................70.0mg
Na2MoO4 ·2H2O ..........................................................36.0mg
NiCl2 ·6H2O.............................................................24.0mg
H3BO3 ..................................................................00.0mg
CuCl2 ·2H2O ...............................................................................2.0mg
HCl (25% solution)....................................................................7.7mL
Preparation of Solution B: Add FeCl2 ·4H2O to 10.0mL of HCl so-
lution. Mix thoroughly. Add distilled/deionized water and bring vol-
ume to 1.0L. Add remaining components. Mix thoroughly. Sparge with
100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Solution C:
Composition per 100.0mL:
NaHCO3 ........................................................................................5.0g
Preparation of Solution C : Add NaHCO3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Sparge with
100% CO2 until saturated, approximately 20 min. Filter sterilize under
100% CO2 into a sterile, gas-tight 100.0mL screw-capped bottle.
Solution D:
Composition per 10.0mL:
Na2 -benzoate.................................................................................0.5g
Preparation of Solution D:  Add Na2 -benzoate to distilled/deion-
ized water and bring volume to 10.0mL. Sparge with N2 . Filter steril-
ize. Store anaerobically.
Solution E:
Composition per liter:
Pyridoxine-HCl........................................................................10.0mg
Thiamine-HCl·2H2O..................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
D -Ca-pantothenate .....................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Lipoic acid.................................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Solution E:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Sparge with 100%
N2 . Filter sterilize.
Solution F:
Composition per liter:
NaOH............................................................................................0.5g
Na2SeO3 ·5H2O...........................................................................3.0mg
Preparation of Solution F: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Sparge with 100%
N2 . Filter sterilize.
Solution G:
Composition per 10.0mL:
Na2S·9H2O....................................................................................0.4g
Preparation of Solution G:  Add Na2S·9H2O to distilled/deionized
water and bring volume to 10.0mL. Sparge with N2 . Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically.
Preparation of Medium:  Add solutions B, C, D, E, F, and G to so-
lution A in that order under N2 gas. Adjust the pH to 7.0.
Use: For the cultivation of Desulfotobacterium spp.