DEV Lactose Peptone MUG Broth

[所属分类:培养基配方] [发布时间:2020-6-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

DEV Lactose Peptone MUG Broth

培养基配方
Composition per liter:
Lactose......................................................................10.0g
Meat peptone ................................................................10.0g
NaCl..........................................................................5.0g
Tryptophan....................................................................1.0g
4-Methylumbelliferyl-β- D -glucuronide.......................................0.1g
Bromocresol Purple ..........................................................0.01g
pH 7.2 ± 0.2 at 37°C
Source: This medium is available from Fluka, Sigma-Aldrich.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into test
tubes that contain an inverted Durham tube in 10.0mL volumes. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use : For the enrichment and titer determination of coliform bacteria in
connection with the bacteriological examination of water. The pres-
ence of E. coli can be demonstrated by fluorescence in the UV and a
positive indole test.