Dextran Agar

[所属分类:培养基配方] [发布时间:2020-6-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dextran Agar

培养基配方
Composition per liter:
Minimal mineral base solution ........................................700.0mL
Agar solution.........................................................200.0mL
Dextran-deoxyglucose solution ........................................100.0mL
pH 4.0 ± 0.2 at 25°C
Minimal Mineral Base Solution:
Composition per 700.0mL:
(NH4)2SO4 .............................................................5.0g
KH2PO4 ................................................................1.5g
CaCl2 .................................................................0.1g
MnSO4 .................................................................0.1g
NaCl...................................................................0.1g
Yeast extract..........................................................0.1g
Preparation of Minimal Mineral Base Solution: Add compo-
nents to distilled/deionized water and bring volume to 700.0mL. Mix
thoroughly. Gently heat and bring to boiling. pH is 4.0 before autoclav-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Agar Solution:
Composition per 200.0mL:
Agar .................................................................10.0g
Preparation of Agar Solution: Add agar to distilled/deionized
water and bring volume to 200.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Dextran-Deoxyglucose Solution:
Composition per 100.0mL:
Dextran...............................................................10.0g
2-Deoxy- D -glucose...................................................0.5g
Preparation of Dextran-Deoxyglucose Solution: Add compo-
nents to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Aseptically combine 700.0mL of sterile
minimal mineral base solution, 200.0mL of sterile agar solution, and
100.0mL of sterile dextran-deoxyglucose solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Lipomyces starkeyi.