Dextran Broth

[所属分类:培养基配方] [发布时间:2020-6-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dextran Broth

培养基配方
Composition per liter:
Minimal mineral base solution ..............................................900.0mL
Dextran-deoxyglucose solution .............................................100.0mL
pH 4.0 ± 0.2 at 25°C
Minimal Mineral Base Solution:
Composition per 900.0mL:
(NH4)2SO4 ...................................................................5.0g
KH2PO4 ......................................................................1.5g
CaCl2 .......................................................................0.1g
MnSO4 .......................................................................0.1g
NaCl.........................................................................0.1g
Yeast extract................................................................0.1g
Preparation of Minimal Mineral Base Solution: Add compo-
nents to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. pH is 4.0 before autoclav-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room tem-
perature.
Dextran-Deoxyglucose Solution:
Composition per 100.0mL:
Dextran ....................................................................10.0g
2-Deoxy- D -glucose..........................................................0.5g
Preparation of Dextran-Deoxyglucose Solution: Add compo-
nents to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Combine 900.0mL of sterile minimal
mineral base solution with 100.0mL of sterile dextran-deoxyglucose
solution. Mix thoroughly. Distribute into sterile flasks or tubes.
Use: For the cultivation and maintenance of Lipomyces starkeyi.