Dichloran Medium Base with Rose Bengal and Selective Supplement

[所属分类:培养基配方] [发布时间:2020-6-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dichloran Medium Base
with Rose Bengal and Selective Supplement

培养基配方
Composition per liter:
Agar......................................................................15.0g
Glucose...................................................................10.0g
Peptic digest of animal tissue ............................................5.0g
KH2PO4 ....................................................................1.0g
MgSO4 .....................................................................0.5g
Rose Bengal..............................................................0.025g
Bromcresol Purple.........................................................0.02g
Dichloran.................................................................0.002
Selective supplement......................................................10.0mL
pH 5.6 ± 0.2 at 25°C
Source: This medium, wthout selective supplement, is available as a
premixed powder from HiMedia.
Selective Supplement:
Composition per 10.0mL:
Chloramphenicol...........................................................0.1mg
Preparation of Selective Supplement: Add chloramphenicol to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. The final pH should be 5.6. Temper in a water
bath at 45°C. Aseptically add 10.0mL sterile selective supplement.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes. This medium is light sensitive and should be stored in a dark,
cool place until used. It is intended for spread plates only.
Use : For the analysis of food samples containing spreader molds, e.g.,
Mucor and Rhizopus. The dichloran and Rose Bengal slow down the
growth of fast-growing fungi, thus allowing the detection of other
fungi with slower growth rates.