Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar) (BAM M183)

[所属分类:培养基配方] [发布时间:2020-6-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dichloran Rose Bengal Chloramphenicol Agar
(DRBC Agar)
(BAM M183)

培养基配方
Composition per liter:
Agar .........................................................................15.0g
Glucose ......................................................................10.0g
Peptone........................................................................5.0g
KH2PO4 ........................................................................1.0g
MgSO4 ·7H2O...................................................................0.5g
Chloramphenicol................................................................0.1g
Dichloran solution............................................................1.0mL
Rose Bengal solution..........................................................0.5mL
pH 5.6 ± 0.2 at 25°C
Dichloran Solution:
Composition per 10.0mL :
Dichloran (2,6-dichloro-4-nitroaniline)........................................0.2g
Preparation of Dichloran Solution: Add dichloran to 10.0mL of
distilled/deionized water. Mix thoroughly.
Rose Bengal Solution:
Composition per 100.0mL :
Rose Bengal....................................................................2.0g
Preparation of Rose Bengal Solution: Add Rose Bengal to
100.0mL of distilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. The final pH should be 5.6. Temper in a water bath at 45°C.
Pour into sterile Petri dishes or distribute into sterile tubes. This medi-
um is light sensitive and should be stored in a dark, cool place until
used. It is intended for spread plates only.
Use : For the analysis of food samples containing spreader molds, e.g.
Mucor and Rhizopus. The dichloran and Rose Bengal slow down the
growth of fast-growing fungi, thus allowing the detection of other
fungi with slower growth rates.