Dichloroacetic Acid Medium No. 2

[所属分类:培养基配方] [发布时间:2020-6-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dichloroacetic Acid Medium No. 2

培养基配方
Composition per liter:
Yeast extract................................................................10.0g
Glucose.......................................................................5.0g
(NH4)2PO4 ....................................................................1.5g
K2HPO4 .......................................................................1.0g
MgSO4 ·7H2O..................................................................0.2g
Fe2(SO4)3 ·5H2O.............................................................0.01g
ZnSO4 ·7H2O................................................................0.002g
2,4-Dichloroacetic acid.....................................................10.0mg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Pseudomonas species.