Dichloromethane Medium for Hyphomicrobium

[所属分类:培养基配方] [发布时间:2020-6-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dichloromethane Medium for Hyphomicrobium

培养基配方
Composition per liter:
K2HPO4 ·3H2O ............................................................4.1g
KH2PO4 ...................................................................1.4g
MgSO4 ·7H2O..............................................................0.2g
(NH4)2SO4 ................................................................0.2g
Dichloromethane (methylene chloride).....................................1.0mL
Trace elements solution..................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
Ca(NO3)2 ................................................................25.0g
FeSO4 ·7H2O..............................................................1.0g
H3BO3 ....................................................................1.0g
MnSO4 ·H2O...............................................................1.0g
Co(NO3)2 ·6H2O..........................................................0.25g
CuCl2 ·2H2O.............................................................0.25g
(NH4)6Mo7O24 ·4H2O......................................................0.25g
ZnCl2 ...................................................................0.25g
NH4VO3 ...................................................................0.1g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Filter sterilize dichloromethane. Add com-
ponents, except dichloromethane, to distilled/deionized water and bring
volume to 999.0mL. Mix thoroughly. Gently heat and bring to boiling. Ad-
just pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile dichloromethane. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Hyphomicrobium species.