Dichotomicrobium thermohalophilum Agar

[所属分类:培养基配方] [发布时间:2020-6-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dichotomicrobium thermohalophilum Agar

培养基配方
Composition per liter:
Agar.......................................................................18.0g
Disodium  DL -malate........................................................1.0g
Yeast extract...............................................................1.0g
Artificial seawater, 3×..................................................960.0mL
Hutner’s basal salts solution ............................................20.0mL
NaHCO3 solution............................................................20.0mL
pH 7.0–7.2 at 25°C
Artificial Seawater, 3 × :
Composition per liter:
NaCl........................................................................70.43g
MgCl2 ·6H2O................................................................31.86g
Na2SO4 .....................................................................11.75g
CaCl2 ·2H2O.................................................................4.35g
NaHCO3 ......................................................................2.88g
KCl..........................................................................1.99g
KBr..........................................................................0.29g
H3BO3 .......................................................................0.08g
Preparation of Artificial Seawater, 3 × :  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Hutner’s Basal Salts Solution:
Composition per liter:
MgSO4 ·7H2O.................................................................29.7g
Nitrilotriacetic acid........................................................10.0g
CaCl2 ·2H2O.................................................................3.335g
FeSO4 ·7H2O.................................................................99.0mg
(NH4)6MoO7O24 ·4H2O ........................................................9.25mg
"Metals 44" .................................................................50.0mL
"Metals 44":
Composition per 100.0mL:
ZnSO4 ·7H2O................................................................1.095g
FeSO4 ·7H2O..................................................................0.5g
Sodium EDTA..................................................................0.25g
MnSO4 ·H2O.................................................................0.154g
CuSO4 ·5H2O................................................................39.2mg
Co(NO3)2 ·6H2O ............................................................24.8mg
Na2B4O7 ·10H2O.............................................................17.7mg
Preparation of “Metals 44”: Add sodium EDTA to distilled/de-
ionized water and bring volume to 90.0mL. Mix thoroughly. Add a few
drops of concentrated H2SO4 to retard precipitation of heavy metal
ions. Add remaining components. Mix thoroughly. Bring volume to
100.0mL with distilled/deionized water.
Preparation of Hutner’s Basal Salts Solution:  Add nitrilotria-
cetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5
with KOH. Add remaining components. Add distilled/deionized water
to 1.0L. Adjust pH to 6.8.
NaHCO3 Solution:
Composition per 20.0mL:
NaHCO3 ......................................................................3.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 20.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except NaHCO3  solu-
tion, to distilled/deionized water and bring volume to 980.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 20.0mL of
sterile NaHCO3  solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation and maintenance of Dichotomicrobium ther-
mohalophilum.