Differential Buffered Charcoal Yeast Extract Agar Base with Selective Supplement

[所属分类:培养基配方] [发布时间:2020-6-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Differential Buffered Charcoal Yeast Extract Agar
Base with Selective Supplement

培养基配方
Composition per liter:
Agar..........................................................................15.0g
ACES buffer ..................................................................10.0g
Yeast extract.................................................................10.0g
Charcoal, activated ...........................................................1.5g
L -Cysteine·HCl ..............................................................0.4g
Ferric pyrophosphate, soluble.................................................0.25g
α-Ketoglutarate...............................................................0.2g
Bromcresol Purple.............................................................0.01g
Bromthymol Blue...............................................................0.01g
Selective supplement.........................................................10.0mL
pH 6.9 ± 0.2 at 25°C
598 Differential Reinforced Clostridial HiVeg Broth Base with Ferric Citrate and Sodium Sulfite
Source: This medium, wthout selective supplement, is available as a
premixed powder from HiMedia.
Selective Supplement:
Composition per 10.0mL:
Vancomycin ...................................................................0.1g
Polymyxin B...........................................................50,000 units
Preparation of Selective Supplement: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL sterile
selective supplement. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the selective isolation and differentiation of Legionella species.