Differential Reinforced Clostridial HiVeg Broth Base with Ferric Citrate and Sodium Sulfite

[所属分类:培养基配方] [发布时间:2020-6-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Differential Reinforced Clostridial HiVeg Broth Base
with Ferric Citrate and Sodium Sulfite

培养基配方
Composition per liter:
Plant extract .............................................................10.0g
Plant peptone..............................................................10.0g
Sodium acetate, hydrated....................................................5.0g
Yeast extract...............................................................1.5g
Glucose ....................................................................1.0g
Starch .....................................................................1.0g
L -Cysteine·HCl............................................................0.5g
Ferric citrate sodium sulfite solution....................................20.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, wthout sodium sulfite and ferric citrate, is
available as a premixed powder from HiMedia.
Ferric Citrate Sodium Sulfite Solution:
Composition per 100.0mL:
Ferric citrate solution..................................................50.0mL
Sodium sulfite solution..................................................50.0mL
Preparation of Ferric Citrate Sodium Sulfite Solution:  Asep-
tically combine 50.0mL of sterile ferric citrate solution and 50.0mL of
sterile sodium sulfite solution. Mix thoroughly. Filter sterilize.
Ferric Citrate Solution:
Composition per 100.0mL:
Ferric citrate............................................................7.0g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize
Sodium Sulfite Solution:
Composition per 100.0mL:
Na2SO3 ...................................................................7.0g
Preparation of Sodium Sulfite Solution:  Add Na2SO3 to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except ferric citrate-so-
dium sulfite solution, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 25°C. Just before using,
aseptically add 20.0mL sterile ferric citrate sodium sulfite solution.
Mix thoroughly. Aseptically distribute into tubes or flasks.
Use:  For the cultivation and enumeration of Clostridium species from
water.