Dihydrolase Broth Base with Arginine

[所属分类:培养基配方] [发布时间:2020-6-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dihydrolase Broth Base with Arginine

培养基配方
Composition per liter:
NaCl........................................................................30.0g
Yeast extract................................................................6.0g
Peptic digest of animal tissue ..............................................5.0g
Glucose......................................................................2.0g
Bromcresol Purple..........................................................0.032g
L -Arginine solution.......................................................50.0mL
pH 6.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Arginine Solution:
Composition per 100.0mL:
L- Arginine.................................................................10.0g
Preparation of Arginine Solution: Add  L- arginine to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except arginine solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat until dissolved. Distribute into screw-capped tubes
in 5.0mL volumes. Autoclave for 15 min at 10 psi pressure–115°C.
Aseptically add 50.0mL sterile arginine solution.
Use: For the differentiation of Gram-negative enteric bacteria based on
the production of arginine dihydrolase.