Dilute Potato Medium (DSMZ Medium 789)

[所属分类:培养基配方] [发布时间:2020-6-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dilute Potato Medium
(DSMZ Medium 789)

培养基配方
Composition per liter:
Glucose ....................................................................1.0g
Na2HPO4 ...................................................................0.12g
Ca(NO3)2 ·4H2O............................................................0.05g
Peptone....................................................................0.05g
Potato decoction.........................................................100.0mL
pH 7.3 ± 0.2 at 25°C
Potato Decoction:
Diced potato..............................................................20.0g
Preparation of Potato Decoction: Add diced potatoes to distilled/
deionized water and bring volume to 1.0L. Boil for 30 min. Filter to re-
move solid potatoes. Bring volume to 1.0L with distilled/deionized wa-
ter.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of various fungi.