Dilute Potato Medium

[所属分类:培养基配方] [发布时间:2020-6-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dilute Potato Medium

培养基配方
Composition per 1090.0mL:
Glucose ....................................................................1.0g
Na2HPO4 ...................................................................0.12g
Ca(NO3)2 ·4H2O)...........................................................0.05g
Peptone....................................................................0.05g
Potato decoction.........................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Potato Decoction:
Composition per liter:
Potato....................................................................20.0g
Preparation of Potato Decoction: Peel and dice potato. Add to
1.0L of distilled/deionized water. Gently heat and bring to boiling.
Continue boiling for 30 min. Filter through Whatman #1 filter paper.
Bring volume of filtrate to 1.0L with distilled/deionized water.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1090.0mL. Mix thoroughly. Adjust pH to
6.8. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use: For the cultivation and maintenance of Rhizobacter daucus.