Dinoflagellate Medium

[所属分类:培养基配方] [发布时间:2020-6-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dinoflagellate Medium

培养基配方
Composition per 1020.0mL:
Seawater solution........................................................1.0L
Basal solution.........................................................20.0mL
pH 7.8 ± 0.2 at 25°C
Seawater Solution:
Composition per 1100.0mL:
Seawater..............................................................1010.0mL
Preparation of Seawater Solution: Add seawater to distilled/de-
ionized water and bring volume to 1100.0mL. Mix thoroughly. Adjust
pH to 7.8. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Basal Solution:
Composition per 100.0mL:
Buffer salts solution..................................................25.0mL
Fe solution ...........................................................25.0mL
Vitamin solution.......................................................25.0mL
Metal solution.........................................................25.0mL
Preparation of Basal Solution: Adjust final pH to 7.8.
Buffer Salts Solution:
Composition per 25.0mL:
Tris-HCl................................................................500.0mg
NaNO3 ..................................................................350.0mg
Sodium glycerophosphate·6H2O ...........................................50.0mg
Preparation of Buffer Salts Solution: Add components to dis-
tilled/deionized water and bring volume to 25.0mL. Adjust ph to 7.8.
Mix thoroughly.
Fe Solution:
Composition per 500.0mL:
Fe(NH4)2(SO4)2 ·6H2O..................................................351.0mg
EDTA...................................................................330.0mg
Preparation of Fe Solution: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly.
Vitamin Solution:
Composition per 25.0mL:
Vitamin B 12 .........................................................10.0μg
Biotin.................................................................5.0μg
Thiamine...............................................................0.5mg
Preparation of Vitamin Solution: Add components to distilled/de-
ionized water and bring volume to 25.0mL. Mix thoroughly.
Metal Solution:
Composition per 25.0mL:
H3BO3 ...............................................................114.0mg
EDTA.................................................................100.0mg
MnSO4 ·4H2O..........................................................16.4mg
FeCl3 ·6H2O...........................................................4.9mg
ZnSO4 ·7H2O...........................................................2.2mg
CoSO4 ·7H2O..........................................................0.48mg
Preparation of Metal Solution : Add components, in the order list-
ed, to distilled/deionized water and bring volume to 25.0mL. Mix thor-
oughly. Adjust pH to 7.5.
Preparation of Basal Solution: Combine 25.0mL buffer salts so-
lution, 25.0mL Fe solution, 25.0mL vitamin solution, and 25.0mL met-
al solution. Adjust pH to 7.8. Mix thoroughly. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Aseptically combine 20.0mL of sterile
basal solution with 1.0L of sterile seawater solution. Mix thoroughly.
Aseptically distribute into sterile, screw-capped tubes or flasks.
Use: For the cultivation of Amphidinium carteri.