Diphasic Blood Agar Base Medium (ATCC Medium 449)

[所属分类:培养基配方] [发布时间:2020-6-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Diphasic Blood Agar Base Medium
(ATCC Medium 449)

培养基配方
Composition per 500.0mL:
Beef........................................................................25.0g
Agar........................................................................10.0g
Neopeptone..................................................................10.0g
NaCl.........................................................................2.5g
Preparation of Medium: Trim beef to remove fat. Add 25.0g of
lean beef to 250.0mL of distilled/deionized water. Gently heat and
bring to boiling. Boil for 2–3 min. Filter through Whatman #2 filter pa-
per. Add agar, neopeptone, and NaCl to filtrate. Bring volume to
500.0mL with distilled/deionized water. Mix thoroughly. Adjust pH to
7.2–7.4. Gently heat and bring to boiling. Autoclave for 20 min at 15
psi pressure–121°C. Cool to 50°–55°C. Add as required to other dipha-
sic blood agars.
Use: As the base medium for diphasic blood agars.