Diphosphothiamine Medium

[所属分类:培养基配方] [发布时间:2020-6-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Diphosphothiamine Medium

培养基配方
Composition per liter:
Proteose peptone...........................................................20.0g
Glucose ...................................................................10.0g
NaCl........................................................................5.0g
Tween™ 40..................................................................0.05g
Diphosphothiamine..........................................................1.0mg
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except diphosphothia-
mine, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat until dissolved. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 1.0mg of diphos-
phothiamine. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use: For the cultivation of Haemophilus piscium.