Diphtheria Virulence HiVeg Agar Base with Tellurite and Diphtheria Virulence Supplement

[所属分类:培养基配方] [发布时间:2020-6-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Diphtheria Virulence HiVeg Agar Base
with Tellurite and Diphtheria Virulence Supplement

培养基配方
Composition per liter:
Plant peptone No.3..........................................................20.0g
Agar........................................................................15.0g
NaCl.........................................................................2.5g
Diptheria virulence supplement............................................200.0mL
Tellurite solution.........................................................50.0mL
pH 7.8± 0.2 at 25°C
Source: This medium, without tellurite or diphtheria virulence sup-
plement, is available as a premixed powder from HiMedia.
Tellurite Solution:
Composition per 100.0mL:
K2TeO3 .....................................................................1.0g
Preparation of Tellurite Solution: Add K2TeO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Caution: Potassium tellurite is toxic.
Diphtheria Virulence Supplement:
Composition per 260.0mL:
Horse serum..............................................................200.0mL
Potassium tellurite solution..............................................60.0mL
Preparation of Diphtheria Virulence Supplement: Aseptical-
ly combine sterile horse serum and sterile tellurite solution. Mix thor-
oughly.
Preparation of Medium: Add components, except tellurite solu-
tion and diphtheria virulence supplement, to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 55–60°C. Aseptically add 2.0mL of sterile
diphtheria virulence supplement and 0.5mL sterile tellurite solution to
each Petri dish. Quickly add 10.0mL sterile Diphtheria Virulence
HiVeg Base Agar to each Petri dish. Before the medium solidifies,
place a filter paper strip saturated with potent diphtheria antitoxin
across the diameter of the plate. Allow the strip to sink to the bottom
of the Petri plate. Inoculate the plate with a heavy inoculum across the
strip.
Use: For the detection of diphtheria toxin producing strains of Coryne-
bacterium diphtheriae. For testing the toxigenicity of Corynebacterium
diphtheriae. The reaction of antitoxin forms the actual basis for the detec-
tion of the diphtheria toxin.