Dorset Egg Medium

[所属分类:培养基配方] [发布时间:2020-6-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dorset Egg Medium

培养基配方
Composition per liter:
Homogenized whole egg ...............................................950.0mL
Glycerol .............................................................50.0mL
pH 6.8–7.4 at 25°C
Source:  This medium is available as a prepared medium from BD Di-
agnostic Systems.
Homogenized Whole Egg:
Composition per liter:
Whole eggs ...........................................................18–24
Preparation of Homogenized Whole Egg:  Use fresh eggs, less
than 1 week old. Scrub the shells with soap. Let stand in a soap solution
for 30 min. Rinse in running water. Soak eggs in 70% ethanol for 15
min. Break the eggs into a sterile container. Homogenize by shaking.
Filter through four layers of sterile cheesecloth into a sterile graduated
cylinder. Measure out 1.0L.
Preparation of Medium: Filter sterilize glycerol. Combine glycer-
ol and homogenized whole egg. Mix thoroughly. Distribute into sterile
screw-capped tubes. Place tubes in a slanted position. Inspissate at
85°C (moist heat) for 45 min.
Use: For the maintenance of Mycobacterium species.