Double-Strength Crude Lactobacillus Medium

[所属分类:培养基配方] [发布时间:2020-6-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Double-Strength Crude Lactobacillus Medium

培养基配方
Composition per 1025.0mL:
Yeast extract (Basamine) .................................................20.0g
Sucrose...................................................................20.0g
Casein hydrolysate........................................................15.0g
Potassium acetate..........................................................3.0g
Histidine·HCl·H2O........................................................2.0g
Ascorbic acid .............................................................1.0g
Pyridoxamine·HCl ......................................................33.0μg
Salts solution A.........................................................20.0mL
Salts solution B..........................................................5.0mL
pH 5.4 ± 0.2 at 25°C
Salts Solution A:
Composition per liter:
K2HPO4 ·3H2O.............................................................16.5g
KH2PO4 ·H2O..............................................................16.5g
Preparation of Salts Solution A:  Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C.
Salts Solution B:
Composition per liter:
MgSO4 ·7H2O...............................................................8.0g
FeSO4 ·7H2O...............................................................0.4g
NaCl.......................................................................0.4g
HCl, concentrated ........................................................0.1mL
MnSO4 ·H2O...............................................................0.1mL

Preparation of Salts Solution B:  Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except salts solution A
and salts solution B, to distilled/deionized water and bring volume to
975.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 20.0mL of sterile salts solution A and 5.0mL
of sterile salts solution B. Mix thoroughly. Adjust pH to 5.4. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Lactobacillus species.