Doyle's Enrichment HiVeg Broth Base with Antibiotic Solution

[所属分类:培养基配方] [发布时间:2020-6-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Doyle's Enrichment HiVeg Broth Base
with Antibiotic Solution

培养基配方
Composition per liter:
Plant hydrolysate .........................................................10.0g
Plant peptone .............................................................10.0g
NaCl........................................................................5.0g
Sodium succinate............................................................3.0g
Yeast extract...............................................................2.0g
Glucose.....................................................................1.0g
L -Cysteine·HCl ...........................................................0.1g
NaHSO3 .....................................................................0.1g
Antibiotic solution.......................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without antibiotic solution, is available as a
premixed powder from HiMedia.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide............................................................0.05g
Vancomycin..............................................................0.015g
Trimethoprim lactate.....................................................5.0mg
Polymyxin B...........................................................200,000U
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add a selective
sterile antibiotic solution. Generally 50.0mL of defibrinated horse
blood is also added as an enrichment. Mix thoroughly.
Use: For the cultivation and enrichment of Campylobacter species
from foods.