DPM Medium (DSMZ Medium 737)

[所属分类:培养基配方] [发布时间:2020-6-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

DPM Medium
(DSMZ Medium 737)

培养基配方
Composition per liter:
Na-propionate ...............................................................1.20g
KH2PO4 .......................................................................1.0g
MgSO4 ·7H2O..................................................................0.1g
CaCl2 ·2H2O.................................................................0.01g
Chelated iron solution.......................................................1.8mL
Trace elements solution .....................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Chelated Iron Solution:
Composition per liter:
Na2 -EDTA.....................................................................7.56g
FeSO4 ·5H2O..................................................................5.54g
Preparation of Chelated Iron Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
H3BO3 ........................................................................2.860
MnCl2 ·4H2O .................................................................1.81g
ZnSO4 ·7H2O..................................................................0.22g
CuSO4 ·5H2O..................................................................0.08g
Na2MoO4 ·4H2O...............................................................0.025g
CoCl2 ......................................................................0.025g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Frankia sp.