DRBC Agar (Dichloran Rose Bengal Chloramphenicol Agar)

[所属分类:培养基配方] [发布时间:2020-6-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

DRBC Agar
(Dichloran Rose Bengal Chloramphenicol Agar)

培养基配方
Composition per liter:
Agar........................................................................15.0g
Glucose.....................................................................10.0g
Peptone .....................................................................5.0g
KH2PO4 ......................................................................1.0g
MgSO4 ·7H2O.................................................................0.5g
Rose Bengal................................................................0.025g
Dichloran..................................................................0.002g
Chloramphenicol solution...................................................10.0mL
pH 5.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Chloramphenicol Solution:
Composition per 10.0mL:
Chloramphenicol.............................................................0.1g
Preparation of Chloramphenicol Solution: Add chlorampheni-
col to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except chlorampheni-
col solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
chloramphenicol solution. Mix thoroughly. Pour into sterile Petri dish-
es or distribute into sterile tubes.
Use: For the isolation, cultivation, and enumeration of yeasts and
molds associated with food spoilage.