Drigalski Litmus Lactose Agar, Modified

[所属分类:培养基配方] [发布时间:2020-6-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Drigalski Litmus Lactose Agar, Modified

培养基配方
Composition per liter:
Agar ......................................................................16.0g
Lactose....................................................................10.0g
Peptic digest of animal tissue.............................................10.0g
Beef extract................................................................4.0g
Bromthymol Blue ...........................................................0.04g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the maintenance of lactic acid bacteria and differentiation of
bacteria on the basis of lactose fermentation. Bacteria that ferment lac-
tose appear as red colonies and others as dark blue-purple colonies.