Dubos Broth

[所属分类:培养基配方] [发布时间:2020-6-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dubos Broth

培养基配方
Composition per liter:
Na2HPO4 ....................................................................2.5g
L- Asparagine...............................................................2.0g
KH2PO4 .....................................................................1.0g
Pancreatic digest of casein.................................................0.5g
Tween™ 80...................................................................0.2g
CaCl2 ·2H2O ..............................................................0.5mg
CuSO4 .....................................................................0.1mg
ZnSO4 ·7H2O...............................................................0.1mg
Ferric ammonium citrate....................................................0.05g
MgSO4 ·7H2O...............................................................0.01g
Bovine serum albumin or bovine serum......................................20.0mL
pH 6.5 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except bovine serum or
bovine serum albumin, to distilled/deionized water and bring volume
to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add sterile bovine serum or bovine serum albumin. Mix
thoroughly. Aseptically distribute into sterile tubes.
Use: For the cultivation of Mycobacterium tuberculosis and other
Mycobacterium species.