Dubos Broth Base with Serum and Glycerol

[所属分类:培养基配方] [发布时间:2020-6-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dubos Broth Base with Serum and Glycerol

培养基配方
Composition per liter:
Na2HPO4 ......................................................................2.5g
L -Asparagine.................................................................2.0g
KH2PO4 .......................................................................1.0g
Casein enzymatic hydrolysate .................................................0.5g
Polysorbate 80................................................................0.2g
Ferric ammonium citrate......................................................0.05g
MgSO4 .......................................................................0.01g
CaCl2 .......................................................................0.5mg
CuSO4 .......................................................................0.1mg
ZnSO4 .......................................................................0.1mg
Glycerol ...................................................................50.0mL
Bovine serum or bovine albumin V............................................20.0mL
pH 6.5 ± 0.2 at 25°C
Source: This medium, without bovine serum or glycerol, is available
as a premixed powder from HiMedia.
Preparation of Medium: Add components, except bovine serum,
to distilled/deionized water and bring volume to 980.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL sterile bo-
vine serum or bovine serum albumin. Mix thoroughly. Aseptically dis-
tribute into sterile tubes.
Use: For the cultivation of Mycobacterium tuberculosis and other
Mycobacterium species.