Dubos Broth with Horse Serum

[所属分类:培养基配方] [发布时间:2020-6-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dubos Broth with Horse Serum

培养基配方
Composition per liter:
Na2HPO4 .........................................................................2.5g
L- Asparagine ...................................................................2.0g
KH2PO4 ..........................................................................1.0g
Pancreatic digest of casein......................................................0.5g
Tween™ 80........................................................................0.2g
CaCl2 ·2H2O....................................................................0.5mg
CuSO4 ..........................................................................0.1mg
ZnSO4 ·7H2O....................................................................0.1mg
Ferric ammonium citrate.........................................................0.05g
MgSO4 ·7H2O....................................................................0.01g
Horse serum....................................................................50.0mL
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse se-
rum. Mix thoroughly. Aseptically distribute into sterile tubes.
Use: For the cultivation and maintenance of Corynebacterium species.