- 定做培养基/定制培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 2025年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
Dubos HiVeg Broth Base with Serum and Glycerol
山东拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
Dubos HiVeg Broth Base with Serum and Glycerol
培养基配方
Composition per liter:
Na2HPO4 .....................................................................2.5g
L -Asparagine................................................................2.0g
KH2PO4 ......................................................................1.0g
Plant hydrolysate ...........................................................0.5g
Polysorbate 80 ..............................................................0.2g
Ferric ammonium citrate.....................................................0.05g
MgSO4 ......................................................................0.01g
CaCl2 ......................................................................0.5mg
CuSO4 ......................................................................0.1mg
ZnSO4 ......................................................................0.1mg
Glycerol ..................................................................50.0mL
Bovine serum or bovine albumin V...........................................20.0mL
pH 6.5 ± 0.2 at 25°C
Source: This medium, without bovine serum or glycerol, is available
as a premixed powder from HiMedia.
Preparation of Medium: Add components, except bovine serum,
to distilled/deionized water and bring volume to 980.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL sterile bo-
vine serum or bovine serum albumin. Mix thoroughly. Aseptically dis-
tribute into sterile tubes.
Use: For the cultivation of Mycobacterium tuberculosis and other
Mycobacterium species.