Dubos Oleic Agar

[所属分类:培养基配方] [发布时间:2020-6-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dubos Oleic Agar

培养基配方
Composition per liter:
Agar.........................................................................15.0g
Na2HPO4 ......................................................................2.5g
KH2PO4 .......................................................................1.0g
L- Asparagine.................................................................1.0g
Pancreatic digest of casein...................................................0.5g
Ferric ammonium citrate......................................................0.05g
MgSO4 ·7H2O.................................................................0.01g
CaCl2 ·2H2O ................................................................0.5mg
CuSO4 .......................................................................0.1mg
ZnSO4 ·7H2O.................................................................0.1mg
Dubos oleic albumin complex.................................................20.0mL
Penicillin solution.........................................................10.0mL
pH 6.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Dubos Oleic Albumin Complex:
Composition per 100.0mL:
Bovine serum albumin, fraction V.............................................5.0g
Oleic acid, sodium salt.....................................................0.05g
NaCl (0.85% solution).....................................................100.0mL
Preparation of Dubos Oleic Albumin Complex: Add bovine
serum albumin and oleic acid to 100.0mL of NaCl solution. Mix thor-
oughly. Filter sterilize.
Penicillin Solution:
Composition per 10.0mL:
Penicillin...............................................................10,000U
Preparation of Penicillin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except Dubos oleic al-
bumin complex and penicillin solution, to distilled/deionized water and
bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add sterile Dubos oleic albumin complex and peni-
cillin solution. Mix thoroughly. Pour into sterile Petri dishes or distrib-
ute into sterile tubes. Allow tubes to cool in a slanted position.
Use: For the isolation of Mycobacterium tuberculosis and determining
its sensitivity to chemotherapeutic agents.